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Index > Protein center > Ptgds(Gene name) > Mouse
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  • Ptgds (Gene name),
  • Prostaglandin-H2 D-isomerase (Protein name ),  PTGDS_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Ptgds;
    Protein name:
    Prostaglandin-H2 D-isomerase;
    Alternative:
    Lipocalin-type prostaglandin-D synthase;Glutathione-independent PGD synthase;Prostaglandin-D2 synthase(L-PGDS;PGD2 synthase;PGDS2);
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
    Subcellular Location:
    Rough endoplasmic reticulum Nucleus membrane Golgi apparatus Cytoplasm perinuclear region Secreted Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
    Protein Attributes:
    Sequence length:
    189
    Sequence:
    50:
    MAALRMLWMG | LVLLGLLGFP | QTPAQGHDTV | QPNFQQDKFL | GRWYSAGLAS | 
    100:
    NSSWFREKKA | VLYMCKTVVA | PSTEGGLNLT | STFLRKNQCE | TKIMVLQPAG | 
    150:
    APGHYTYSSP | HSGSIHSVSV | VEANYDEYAL | LFSRGTKGPG | QDFRMATLYS | 
    189:
    RTQTLKDELK | EKFTTFSKAQ | GLTEEDIVFL | PQPDKCIQE
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    String:
    UniGene:
    SMR:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167d
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167m
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167b
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    E0167p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    U0167r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    P0167Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    P0167Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Polyclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Cat.:
    P0167Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Monoclonal Antibody for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase
    Protein for Mouse Prostaglandin-H2 D-isomerase

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.3%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of PTGDS into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    98

    92-102

    Plasma

    99

    96-104

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGDS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    92-103%

    92-104%

    94-102%

    EDTA plasma(n=5)

    91-106%

    93-105%

    96-101%

    95-100%

    heparin plasma(n=5)

    94-101%

     

    91-104%

    95-102%

    93-105%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Developmental expression of murine Beta-trace in embryos and adult animals suggests a function in maturation and maintenance of blood-tissue barriers."
      Hoffmann A. , Baechner D. , Betat N. , Lauber J. , Gross G.
      Dev. Dyn.207:332-343(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY
      strain: NMRI.
      tissue: Brain.
    2. 2.
      Hoffmann A. , Steinert P. , Lauber J. , Gross G.
      Submitted (1997-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
    3. 3.
      "Isolation of putative prostaglandin D synthetase from mouse choroid plexus."
      Kita H. , Kawamoto S. , Okubo K. , Matsubara K.
      Submitted (1997-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      strain: C57BL/6.
      tissue: Brain.
    4. 4.
      "Lack of tactile pain (allodynia) in lipocalin-type prostaglandin D synthase-deficient mice."
      Eguchi N. , Minami T. , Shirafuji N. , Kanaoka Y. , Tanaka T. , Nagata A. , Yoshida N. , Urade Y. , Ito S. , Hayaishi O.
      Proc. Natl. Acad. Sci. U.S.A.96:726-730(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY
      strain: 129/Sv.
      tissue: Liver.
    5. 5.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Small intestine.
    6. 6.
      Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
      Submitted (2005-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Eye.
    8. 8.
      "Prostaglandin D synthase gene is involved in the regulation of non-rapid eye movement sleep."
      Pinzar E. , Kanaoka Y. , Inui T. , Eguchi N. , Urade Y. , Hayaishi O.
      Proc. Natl. Acad. Sci. U.S.A.97:4903-4907(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    9. 9.
      "Pronounced eosinophilic lung inflammation and Th2 cytokine release in human lipocalin-type prostaglandin D synthase transgenic mice."
      Fujitani Y. , Kanaoka Y. , Aritake K. , Uodome N. , Okazaki-Hatake K. , Urade Y.
      J. Immunol.168:443-449(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY
    10. 10.
      "Perineuronal oligodendrocytes protect against neuronal apoptosis through the production of lipocalin-type prostaglandin D synthase in a genetic demyelinating model."
      Taniike M. , Mohri I. , Eguchi N. , Beuckmann C.T. , Suzuki K. , Urade Y.
      J. Neurosci.22:4885-4896(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    11. 11.
      "Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis."
      Gerena R.L. , Eguchi N. , Urade Y. , Killian G.J.
      J. Androl.21:848-854(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    12. 12.
      "Dexamethasone induces lipocalin-type prostaglandin D synthase gene expression in mouse neuronal cells."
      Garcia-Fernandez L.F. , Iniguez M.A. , Eguchi N. , Fresno M. , Urade Y. , Munoz A.
      J. Neurochem.75:460-470(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION BY DEXAMETHASONE
    13. 13.
      "Structural basis of multi-functional lipocalin-type prostaglandin D synthase."
      RIKEN structural genomics initiative (RSGI)
      Submitted (2006-10) to the PDB data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 24-189
    14. 14.
      "NMR solution structure of lipocalin-type prostaglandin D synthase: evidence for partial overlapping of catalytic pocket and retinoic acid-binding pocket within the central cavity."
      Shimamoto S. , Yoshida T. , Inui T. , Gohda K. , Kobayashi Y. , Fujimori K. , Tsurumura T. , Aritake K. , Urade Y. , Ohkubo T.
      J. Biol. Chem.282:31373-31379(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 25-189;FUNCTION;CATALYTIC ACTIVITY;DOMAIN
    15. 15.
      "Structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase."
      Kumasaka T. , Aritake K. , Ago H. , Irikura D. , Tsurumura T. , Yamamoto M. , Miyano M. , Urade Y. , Hayaishi O.
      J. Biol. Chem.284:22344-22352(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-189 OF MUTANT ALA-65 IN COMPLEX WITH RETINOIC ACID;FUNCTION;CATALYTIC ACTIVITY;MUTAGENESIS OF SER-45; CYS-65; THR-67 AND SER-81;BIOPHYSICOCHEMICAL PROPERTIES;DISULFIDE BOND;DOMAIN
    16. 16.
      "Structural analysis of lipocalin-type prostaglandin D synthase complexed with biliverdin by small-angle X-ray scattering and multi-dimensional NMR."
      Miyamoto Y. , Nishimura S. , Inoue K. , Shimamoto S. , Yoshida T. , Fukuhara A. , Yamada M. , Urade Y. , Yagi N. , Ohkubo T. , Inui T.
      J. Struct. Biol.169:209-218(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 25-189 IN COMPLEX WITH BILIVERDIN;FUNCTION;DOMAIN
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